Перенос 3-х дневок и бластоцист.

Все что мы об этом знаем, или хотим узнать

Модераторы: ПРИМус, tatanka

Сообщение Леля_mama » 25 ноя 2010, 22:45

Лилит, а сюда про перенос эмбрионов. Твои сообщения тоже потом перенесу.
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Сообщение Lilit » 26 ноя 2010, 04:20

Леля, я прочитала полный текст статьи Вайсмана, которые утверждали, что перенос бластоцист имеет смысл только в группе пациентов с хорошим прогнозом. Вот как они описали пациенток с хорошим прогнозом, кому имеет смысл растить до бластиков

It appears that young patients, without multiple previous failed IVF attempts, who produce multiple oocytes and good-quality cleavage-stage embryos, are the best candidates for blastocyst culture and transfer. Obviously, this is also the group at highest risk for multiple gestations, and therefore the ability to restrict the number of blastocysts transferred without compromising the chances for pregnancy in this selective good prognosis group is a major advantage (Gardner et al., 2000).
-молодые пациентки
-без множетвенных безуспешных попыток
-у кого много ооцитов получают
-у кого на 3 день много хороших эмбрионов
-таким пациенткам с хорошим прогнозам предлагается растить до бластиков и перносить 1 бластоцисту, чтобы снизить риск многоплодия
Также они обсуждают, что каждая группа по совему опыту должна утсанвливать ту группу пациентов, кому походит культивирование до бластиков. Потмоу что результаты варируют ои группы к группе.

For example, the Brussels group (Papanikolaou et al., 2005) has recently reported that a threshold of four good embryos on the third day of embryo culture appears to indicate that the patient will benefit from embryo transfer at the blastocyst stage, and this group have a better chance of achieving a live delivery than with cleavage-stage embryo transfer. Здесь пишут, что брюссельцы сообщали, что пациентки, у которых на 3 день есть как минимум 4 хороших эмбриона, имеют более высокий шанс беременности и живорождения, если им культивировать и переносить бластоцисты.

At the current status of knowledge, it is premature to assume that failure to reach the blastocyst stage indicates that the embryo never had implantation potential (Racowsky et al., 2000; Alper et al., 2001). While only a fraction of cleavage-stage embryos reaches the blastocyst stage, it is currently unknown how many of the other embryos could potentially result in a live birth if transferred at the cleavage stage. Therefore, the incorporation of blastocyst-stage transfer into routine clinical practice should be done on a selective clinic- and patient-specific basis, and should certainly not be offered to all patients.
В заключение они написали, что на данном этапе преждевременно предполагать, что если 3хдневки не смогли дорастить до бластиков в культуре, то значит они не имели совершенно никакого имплантационного потенциала.

это статья от 2008 года, не знаю, может что поменялось с тех пор
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Сообщение Ромаshka » 26 ноя 2010, 11:02

девочки, спасибо!!!!!! такаие важные вопросы поднимаете!!!!! :nata-smilwithflowers:
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Сообщение Lilit » 05 дек 2010, 02:15

FROZEN THAWED EMBRYO TRANSFER VERSUS FRESH EMBRYO TRANSFER
I.V. Zorina, E.A. Osina, V.P. Apryshko, S.A. Yakovenko.
“Altravita” IVF Clinic, Moscow, Russia C.I.S

Objective:
Controlled ovarian hyperstimulation has been shown to advance endometrial maturation and adversely affects implantation in ART. It has been reported that there is a better embryoendometrium synchrony in frozen thawed embryo transfer cycles than fresh embryo transfer cycles. The purpose of investigation was to study whether frozen thawed embryo transfer (FET) instead of fresh embryo transfer in a controlled ovarian hyperstimulation (COH) cycle improves the in vitro fertilization-ET clinical pregnancy rate and to evaluate the
most effective endometrial preparation for women undergoing transfer with frozen embryos.
Methods:
We retrospectively analyzed the clinical data of 664 cycles with embryo transfer of two fresh or frozen good quality blastocysts, including 330 fresh ET cycles after COH (Group A) and 38 FET in spontaneous ovulatory cycles (Group B) and 296 FET cycles in which the endometrium is artificially prepared by estrogen and progesterone hormones, without a gonadotropin releasing hormone agonist (GnRHa) (Group C).
Selection Criteria:
We included the cycles with embryo transfer of two fresh or frozen good quality blastocysts. The chosen method of embryo cryopreservation was vitrification according to Kuwayama.
Results:
There were 26 (68.4%) ongoing pregnancies in FET group B and 154 (52.02%) ongoing pregnancies in FET group C compared with 138 (41.8%) ongoing pregnancies in fresh ET group A.
Conclusions:
FET can be performed instead of fresh ET to improve the outcome of ART in highly selected patients with good quality blastocyst. The results suggest the superiority of the natural cycle as compared with the cycles in which the endometrium is artificially prepared by estrogen and progesterone hormones.
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Сообщение Lilit » 05 дек 2010, 02:20

COMPREHENSIVE CHROMOSOME SCREENING SIGNIFICANTLY IMPROVES IMPLANTATION RATES FOLLOWING FROZEN BLASTOCYST TRANSFER.

J. M. Stevens, E. S. Surrey, D. A. Minjarez, R. L. Gustofson, W. B. Schoolcraft, M. G. Katz-Jaffe Colorado Center for Reproductive Medicine, Lone Tree, CO

OBJECTIVE: Blastocyst comprehensive chromosome screening (CCS) of all 23 pairs of chromosomes has demonstrated promising clinical results including higher implantation rates per blastocyst transferred. The aim of this study was to evaluate the contribution of a frozen blastocyst transfer (FBT) incorporating a non-stimulated endometrium, against the transfer of euploid blastocysts, in relation to the observed high implantation rates.
DESIGN: Retrospective analysis of 232 'Freeze All' IVF cycles from May 2007 to March 2010.
MATERIALS AND METHODS: Blastocyst 'Freeze All' IVF cycles (n=232) were grouped accordingly: Group A = FBT with CCS (n=184) and Group B = FBT only due to OHSS risk (n=48). CCS was performed on trophectoderm biopsies by mCGH (Reprogenetics) or SNP microarray (RMA-NJ) prior to vitrification. Blastocysts were warmed and transferred in a subsequent FBT based on euploid chromosomes only for Group A and blastocyst morphology grading for Group B.
RESULTS: Ovarian reserve parameters and baseline cycle characteristics were shown to be similar between these 'Freeze All' patient groups. High blastocyst survival following vitrification and warming was also comparable, Group A=335/344 (97.38%) and Group B=116/121 (95.9%, ns). However, there were very significant differences in relation to maternal age (A=37.55±3.25 v. B=34.85±4.28 yrs, P<0.0001), number of blastocysts transferred (A=1.8±0.62 v. B=2.27±0.84, P<0.0001) and implantation rate with fetal cardiac activity (A=60.84% v. B=44.0%, P<0.01). The combination of these significant parameters resulted in similar clinical pregnancy rates between the groups (A=73.4% and B=72.9%, ns).
CONCLUSION: Blastocyst vitrification with a follow up FBT results in high clinical pregnancy rates and is a valuable technique to clinical ART. Despite being from a group with a poorer prognosis incorporation of blastocyst CCS for embryo selection significantly improved the implantation rate per blastocyst transferred. An appropriately designed RCT is underway to resolve this question.
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Сообщение Lilit » 05 дек 2010, 02:27

BLASTOMERE CLEAVAGE SYNCHRONICITY IN NONINVASIVE SELECTION OF EUPLOID EMBRYOS WITH HIGH IMPLANTATION POTENTIAL
V.P. Apryshko, S.A. Yakovenko, E.A. Seregina, E.V. Yutkin.
“Altravita” IVF Clinic, Moscow, Russia C.I.S

Introduction:
We have developed a new noninvasive method of euploid embryo selection based on the hypothesis that embryos with synchronously cleaving blastomeres are mostly euploid with greater likelihood of implantation, and that aneuploid embryos tend to cleave asynchronously. It was reported that chromosome segregation during meiosis or mitosis in human does not occur simultaneously and randomly. The time period necessary for
complete chromosome segregation depends on the number of chromosomes; it varies for aneuploid and euploid cells of the same species. We hypothesized that the cell cycle duration also depends on the ploidy of the cells of the same type and same species. Given that aneuploidy of blastomeres leads to embryo mosaicism, we assumed that blastomeres of mosaic embryos do not cleave synchronously due to the different cell cycle duration
of cells with varying number of chromosomes. Blastomere division synchronicity in preimplantation embryos might become an efficient noninvasive criterion for selecting euploid embryos for embryo transfer, partially replacing preimplantation genetic diagnosis (PGD) for aneuploidy. The aim of this study is to prove our hypothesis that embryos with synchronously cleaving blastomeres have higher probabilities to implant and aneuploid embryos cleave mostly asynchronously.
Materials and Methods:
A mathematical model was developed to calculate the probability of implantation for an individual embryo based on the pregnancy outcome of 6032 in vitro fertilization (IVF) cycles with one or two embryos transferred, which took place between 2004 and 2010. Cleavage was deemed synchronous if the embryo had 4 blastomeres on Day 2 and 8 cells on Day 3 of cultivation. We calculated the implantation probability for embryos with 4, 5, 6, 7, 8, 9, 10, and 12 blastomeres on Day 3, as well as the probability of singleton and multiple pregnancies and the
selection of the pair of embryos best for transfer. The cleavage synchronicity rate was calculated for each type of aneuploidy.
Results:
Synchronously cleaving embryos with 8 blastomeres on Day 3 of development had the highest probability to implant (0.366±0.012). The implantation probability of the embryos with 4 cells on Day 3 and synchronous cleavage was 0.301±0.034.
The 6-cell Day 3 embryos with asynchronously cleavage were characterized by the markedly lower probability of implantation (0.197±0.019); embryos with speediest cleavage (attaining 12 blastomeres on Day 3) had the lowest implantation probability (0.118±0.062). PGD for aneuploidy of 278 embryos demonstrated correlation between cell division synchronicity and aneuploidy of embryos; 80% of embryos with aneuploidy detected by PGD
divided asynchronously, whereas 53% of euploid embryos divided synchronously. Thus, PGD has confirmed our hypothesis that embryos with synchronously cleaving blastomeres tend to be euploid with good implantation prognosis. The link between the synchronicity of embryo cleavage and aneuploidy rate provides us with a instrument for noninvasive selection of embryos in order to minimize fetal abnormalities and decrease the
frequency of multiple pregnancies.
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Сообщение Леля_mama » 02 янв 2011, 23:43

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Сообщение Julya » 19 июн 2011, 08:05

Девочки, ну вот зачем на английском ? Форум русскоговорящий у нас... Так хотелось получить инфу по названию темы - перенос 3-х дневок и бластоцист, а вот не судьба... Пошла в яндекс... :crying-yellow:
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Сообщение Леля_mama » 19 июн 2011, 23:06

Julya74 писал(а):Девочки, ну вот зачем на английском ? Форум русскоговорящий у нас... Так хотелось получить инфу по названию темы - перенос 3-х дневок и бластоцист, а вот не судьба... Пошла в яндекс... :crying-yellow:

Это статьи научные. Перевод был бы не корректным. Каждый переводит сам. Вы же можете воспользоваться авто переводчиком в том же Яндексе.
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